RESUMO
This study compares the effects of human antiphospholipid (aPL) and anti-P-ribosomal (anti-P) IgG and control IgG on the brain. Intracerebroventricular (ICV) injected aPL mice (exAPS) displayed specific hyperactivity compared to anti-P-injected (exSLE) and control mice. In contrast ICV injected anti-P-injected mice specifically displayed depression-like behavior and olfactory impairment compared to the other 2 groups. Both anti-P and aPL injected mice were impaired in the passive avoidance test compared to controls. The distinct cognitive effects of the 2 pathogenic antibodies argue for a specific and differential direct action of these autoantibodies on the brain in clinical disease.
Assuntos
Anticorpos Antifosfolipídeos/toxicidade , Depressão/induzido quimicamente , Hipercinese/induzido quimicamente , Imunoglobulina G/toxicidade , Transtornos do Olfato/induzido quimicamente , Proteínas Ribossômicas/imunologia , Animais , Síndrome Antifosfolipídica/sangue , Síndrome Antifosfolipídica/imunologia , Aprendizagem da Esquiva/efeitos dos fármacos , Aprendizagem da Esquiva/fisiologia , Depressão/imunologia , Depressão/fisiopatologia , Modelos Animais de Doenças , Feminino , Humanos , Hipercinese/imunologia , Injeções Intraventriculares , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Transtornos do Olfato/imunologia , Desempenho Psicomotor/efeitos dos fármacos , Limiar Sensorial/efeitos dos fármacos , Olfato/efeitos dos fármacos , Olfato/imunologiaRESUMO
UNLABELLED: Systemic lupus erythematosus (SLE) is an autoimmune disease associated with more than 100 different autoantibodies, some of which may be associated with specific neuropsychiatric (NPSLE) manifestations. Injection of anti-P ribosomal antibodies (anti-P) directly to the brain ventricles of mice induces depression manifested by increased immobility time in the forced swim test (FST). METHODS: Mice were injected intracerebroventricularily (ICV) with affinity-purified human anti-P antibodies or normal commercial IgG as control. Mice were examined for depression by the forced swimming test (FST) and for olfactory function by the smell threshold test. Treatments included the antidepressant drug fluoxetine or aroma therapy by exposure to lemon or cinnamon odor. RESULTS: Mice injected with anti-P developed depression-like behavior, which improved significantly upon treatment with fluoxetine. Depressed mice had a significant deficit in olfactory function which was not reversed by fluoxetine. Exposure of anti-P-injected mice to lemon odor was associated with some improvement of the immobility time, a measure of depression. CONCLUSIONS: ICV injection of anti-P induces both depression-like behavior and impaired olfactory function in mice. Fluoxetine and possibly lemon odor exposure improve depressive behavior in these mice.